A new method suitable for single nucleotide polymorphism (SNP) detection using differential oligonucleotide probe extension has been developed. Sulfur-linked laser-cleavable trityl labels are implemented in this protocol. The method is based on mass spectrometry and utilizes a single surface for affinity purification of extended probes and matrix-independent desorption–ionization of the cleavable labels. The usefulness of this method for SNP genotyping is demonstrated.
Original text © Methods in Molecular Biology 578 (2009) p.345-361